Sunday, 18 October 2015

Molecular mechanism research on simultaneous therapy of brain and heart based on data mining and network analysis


Molecular mechanism research on simultaneous therapy of brain and heart based on data mining and network analysis


Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(1):91-8
Authors: Chen D, Lu P, Zhang FB, Tang SH, Yang HJ
Abstract
OBJECTIVE: The theory of treating heart and brain simultaneously is from the theory of traditional Chinese medicine, and there aren't enough explanations for this theory from the perspective of molecular mechanism. As one successful case of this theory, the Chinese medicine formula--Buchang Naoxintong can achieve the goal of treating coronary heart disease and stroke at the same time. To illustrate the mechanism of the theory of treating heart and brain simultaneously, it is necessary to find out the molecular mechanism of this formula.
METHOD: Using the network analysis method, together with two data mining methods-clustering and apriori algorithm, the frequent gene combinations interfered by the chemicals of the formula based on the protein-protein interaction networks related with coronary heart disease and stroke disease were figured out respectively. To find out the molecular mechanism of the theory of treating heart and brain simultaneously, the results got from two diseases were compared and analyzed.
RESULT: Based on comparing two results from these two different diseases, the mechanism of the theory of treating heart and brain simultaneously was explained from molecular level by finding out key genes targeted by the components of this formula for both diseases and some particular genes interfered by the components for each disease. In addition, genes interfered indirectly by the chemicals for different diseases were found out based on the protein-protein interaction network.
CONCLUSION: It can help to explain the molecular mechanism of the theory by our methods. By finding out the molecular mechanism of this theory, it can promote the progress of combination of Chinese traditional and Western medicine.
PMID: 23596883 [PubMed - indexed for MEDLINE

Sunday, 11 October 2015

Evaluation of the antibacterial potential of Petroselinum crispum and Rosmarinus officinalis against bacteria that cause urinary tract infections.



Braz J Microbiol. 2013;44(3):829-34
Authors: Petrolini FV, Lucarini R, de Souza MG, Pires RH, Cunha WR, Martins CH

Abstract
In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The crude hydroalcoholic extract of R. officinalis displayed in vitro activity against Gram-positive bacteria, with satisfactory MBC for the clinical isolate S. saprophyticus. The fractions and the pure compound rosmarinic acid did not furnish promising results for Gram-negative bacteria, whereas fractions 2, 3, and 4 gave encouraging results for Gram-positive bacteria and acted as bactericide against S. epidermidis as well as E. faecalis (ATCC 29212) and its clinical isolate. R. officinalis led to promising results in the case of Gram-positive bacteria, resulting in a considerable interest in the development of reliable alternatives for the treatment of urinary infections.

Friday, 20 September 2013

Advances in studies on toxicity of aconite

Advances in studies on toxicity of aconite.
Zhongguo Zhong Yao Za Zhi. 2013 Apr;38(8):1126-9
Authors: Chen RC, Sun GB, Zhang Q, Ye ZG, Sun XB
Abstract
Aconite has the efficacy of reviving yang for resuscitation, dispelling cold and relieving pain, which is widely used in clinic, and shows unique efficacy in treating severe diseases. However, aconite has great toxicity, with obvious cardio-toxicity and neurotoxicity. Its toxicological mechanism main shows in the effect on voltage-dependent sodium channels, release of neurotransmitters and changes in receptors, promotion of lipid peroxidation and cell apoptosis in heart, liver and other tissues. Aconite works to reduce toxicity mainly through compatibility and processing. Besides traditional processing methods, many new modern processing techniques could also help achieve the objectives of detoxification and efficacy enhancement. In order to further develop the medicinal value of aconite and reduce its side effect in clinical application, this article gives comprehensive comments on aconite's toxicity characteristics, mechanism and detoxification methods on the basis of relevant reports for aconite's toxicity and the author's experimental studies.

Thursday, 19 September 2013

Regulatory T-cell modulation by green tea in chronic lymphocytic leukemia.
Int J Immunopathol Pharmacol. 2013 Jan-Mar;26(1):117-25
Authors: D'Arena G, Simeon V, De Martino L, Statuto T, D'Auria F, Volpe S, Deaglio S, Maidecchi A, Mattoli L, Mercati V, Musto P, De Feo V
Abstract
Regulatory T cells (Tregs) are considered to be key immunomodulatory cells of the immune system and are increased in chronic lymphocytic leukemia (CLL). Rai stage 0 identifies patients with early stage CLL for which there is no effective intervention at the present time and a "wait and see" policy is usually adopted.