Chemopreventive properties of raisins originating from Greece in colon cancer cells.
Food Funct. 2012 Dec 5;
Authors: Kountouri AM, Gioxari A, Karvela E, Kaliora AC, Karvelas M, Karathanos VT
Colorectal cancer is one of the major causes of cancer-related
mortality in humans in both developed and developing countries. Dietary
patterns influence the risk of colon cancer development, while
plant-derived foods have gained great interest, due to the high content
of antioxidants. Corinthian raisins (Currants, CR) and Sultanas (S)
(Vitis vinifera L., Vitaceae) are dried vine fruits produced in Greece
with many culinary uses in both the Mediterranean and the Western
In the present study, we investigated the effects of CR and S
on human colon cancer cells. Methanol extracts of CR and S were used at
different concentrations. The total polyphenol content and anti-radical
activity were measured by Folin-Ciocalteu and DPPH, respectively.
Antioxidant, anti-inflammatory and anti-proliferative effects on HT29
cell culture were evaluated.
All extracts exhibited DPPH˙ scavenging
activity in a dose-dependent manner. Both products suppressed cell
proliferation, while the levels of glutathione and cyclooxygenase 2 were
significantly decreased. A significant reduction in IL-8 levels and
NF-kappaB p65 activation was also observed. Both antioxidant and
anti-inflammatory effects were dependent on the duration of exposure.
Results indicate that the methanol extracts of CR and S exhibit
anti-radical activity in vitro, as well as cancer preventive efficacy on
colon cancer cells, with S having slightly higher activity. The
beneficial properties of these unique dried grapes are attributed to
their high content of phenolic compounds.