Wednesday, 31 July 2013

Mistletoe extract for treatment of urological tumours

Mistletoe extract for treatment of urological tumors.
Urologe A. 2012 Dec;51(12):1656-62
Authors: Suttmann H, Saar M, Ohlmann CH, Stöckle M, Kamradt J
Abstract
In Germany misteltoe extract is one of the most commonly used complementary therapeutic strategies in oncology. There are anthroposophical as well as phytotherapeutic concepts to explain the potential mechanism of action; however, the oncological and uro-oncological literature lacks definitive proof to support recommendations on which is the most effective drug, the optimal dose, a clear indication or its efficacy. Weighting the current data, potential side effects and contraindications, the application of mistletoe extract in daily uro-oncological practice requires careful consideration of the indications in the context of a detailed patient informed consent and request for this unique therapeutic modality

Tuesday, 30 July 2013

Study on improving blood flow with Korean red ginseng substances using digital infrared thermal imaging and Doppler sonography: randomized, double blind, placebo-controlled clinical trial with parallel design

Study on improving blood flow with Korean red ginseng substances using digital infrared thermal imaging and Doppler sonography: randomized, double blind, placebo-controlled clinical trial with parallel design.
J Tradit Chin Med. 2013 Feb;33(1):39-45
Authors: Kang J, Lee N, Ahn Y, Lee H
Abstract
OBJECTIVE: To examine the efficacy of Korean red ginseng for improving blood flow in healthy people.
METHODS: Participants were randomized and treated with 1500 mg of Korean red ginseng extract or placebo for 8 weeks. The effect of Korean red ginseng was evaluated by digital infrared thermal images, and Doppler sonography, and blood test.
RESULTS: Forty subjects completed the protocol. Imbalance in local thermal distribution was significantly decreased in the Korean red ginseng group confirmed by digital infrared thermal images. Doppler sonography showed no significant change in maximum and average rates of blood circulation in single or complex areas. Blood analyses for coagulation and lipid metabolism factors revealed no significant changes. No abnormal reactions to the Korean red ginseng were observed.
CONCLUSION: Digital infrared thermal imaging showed that the temperature deviation in the whole body decreased safely in the Korean red ginseng group, which mitigated the body-temperature imbalance. This result suggests that the Korean red ginseng improves blood circulation in the human body.
PMID: 23596810 [PubMed - indexed for MEDLINE

Monday, 29 July 2013

Phytotherapy of benign prostate syndrome and prostate cancer: better than placebo

Phytotherapy of benign prostate syndrome and prostate cancer: better than placebo.
Urologe A. 2012 Dec;51(12):1674-82
Authors: Wehrberger C, Dreikorn K, Schmitz-Dräger BJ, Oelke M, Madersbacher S
Abstract
In some countries plant extracts have belonged to the most popular drugs for the treatment of the benign prostatic syndrome (BPS) for decades; however, only few of the large number of published studies meet the criteria of the WHO benign prostatic hyperplasia (BPH) consensus conference. The few placebo-controlled long-term (study period >6 months) studies suggest a positive effect of some extracts (saw palmetto fruit, β-sitosterol, urtica, rye grass and a saw palmetto/urtica combination) on lower urinary tract symptoms (LUTS), urinary flow rate, post-void residual volume but effects on prostate volume or prostate-specific antigen (PSA) were only inconsistently demonstrable.

Sunday, 28 July 2013

Food allergy

Food allergy prevalence: new possibilities for therapy and prevention.
Wien Med Wochenschr. 2012 Dec;162(23-24):519-24
Authors: Ma Y
Abstract
Food allergy is an important clinical problem of increasing prevalence worldwide. Immunoglobulin E (IgE)-mediated allergic responses are the most widely recognized form of food allergy. The prevalence of food allergy is influenced by country, age, culture, and dietary habits. Strategies for the prevention of food allergy have been extensively studied. There is currently no standard treatment for food allergy and allergen-specific immunotherapy has been hindered by severe side effects in the past. A mutated recombinant major apple allergen is clinically hypoallergenic, which paves the way toward safer immunotherapy for the treatment of food-allergic patients.Traditional Chinese medicine (TCM) is one of the oldest medical practices in the world. A Chinese Food Allergy Herbal Formula-2 (FAHF-2) has been used as a therapy for food allergy patients. FAHF-2 was shown to be remarkably effective against food anaphylaxis in an animal model and in human clinical trial with the potential to be a long-lasting therapy.
PMID: 23188465 [PubMed - indexed for MEDLINE

Saturday, 27 July 2013

Immunomodulating activities of cultivated maitake medicinal mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (higher Basidiomycetes) on peripheral blood mononuclear cells.
Int J Med Mushrooms. 2012;14(4):377-83
Authors: Svagelj M, Berovic M, Gregori A, Wraber B, Simcic S, Boh B
Abstract
Grifola frondosa is a culinary-medicinal mushroom that contains several physiologically active compounds, of which polysaccharides, specifically β-glucans, are known to possess immunomodulating properties. Its extracts are studied for application as adjuncts for chemotherapy, and experiments in animal models support the use of this mushroom for cancer treatment. The effect of extracts obtained from mushrooms cultivated on different substrates and their capacity of inducing the secretion of cytokines from human peripheral blood mononuclear cells were studied. The activity of extracts at concentrations 12.5, 100, and 200 μg/mL on induction of TNF-α, IFN-γ, and IL-12 was screened. Two extracts from substrates fortified with olive oil press cakes showed appreciable activity and induced the secretion of TNF-α, IL-12, and INF-γ. The extracts differed from the others in the amount of sugar, protein, and β-glucans, which can explain their higher activity. Present results show that different substrates and different source materials can reasonably modify the bioactivity of cultivated G. frondosa.
PMID: 23510175 [PubMed - indexed for MEDLINE]

Friday, 26 July 2013

Nurses and benzodiazepine withdrawal

Analysis of benzodiazepine withdrawal program managed by primary care nurses in Spain.
BMC Res Notes. 2012;5:684
Authors: Lopez-Peig C, Mundet X, Casabella B, del Val JL, Lacasta D, Diogene E
Abstract
BACKGROUND: Benzodiazepine (BZD), the long-term treatment of which is harmful for cognitive function, is widely prescribed by General Practitioners in Spain. Based on studies performed in other countries we designed a nurse-led BZD withdrawal program adapted to Spanish Primary Care working conditions.
RESULTS: A pseudo-experimental (before-after) study took place in two Primary Care Centres in Barcelona. From a sample of 1150 patients, 79 were identified. They were over 44 years old and had been daily users of BZD for a period exceeding six months. Out of the target group 51 patients agreed to participate. BZD dosage was reduced every 2-4 weeks by 25% of the initial dose with the optional support of Hydroxyzine or Valerian.

Thursday, 25 July 2013

Deoxypodophyllotoxin: A promising therapeutic agent from herbal medicine.

Deoxypodophyllotoxin: A promising therapeutic agent from herbal medicine.
J Ethnopharmacol. 2013 Jun 20;
Authors: Meyada K, Zhen-Zhou J, Lu-Yong Z
Abstract
BACKGROUND: Recently, biologically active compounds isolated from plants used in herbal medicine have been the center of interest. Deoxypodophyllotoxin (DPT), structurally closely related to the lignan podophyllotoxin, is a potent antitumor and anti-inflammatory agent. However, DPT has not been used clinically yet. Also, DPT from natural sources seems to be unavailable. Hence, it is important to establish alternative resources for the production of such lignan; especially that it is used as a precursor for the semi-synthesis of the cytostatic drugs etoposide phosphate and teniposide

Wednesday, 24 July 2013

Essential factors of Sui Zheng Shi Liang strategy in type 2 diabetes treatment assessed by questionnaire.

Essential factors of Sui Zheng Shi Liang strategy in type 2 diabetes treatment assessed by questionnaire.
J Tradit Chin Med. 2013 Feb;33(1):27-33
Authors: Lian F, Chen X, Zhao L, Xu L, Tong X
Abstract
OBJECTIVE: Sui Zheng Shi Liang strategy (regulating prescription dosage with different syndromes) is an important part of syndrome differentiation and treatment in Traditional Chinese Medicine (TCM). Questionnaires were given to doctors and patients to study the essential factors (indicators and timing) of Sui Zheng Shi Liang strategy in the treatment process of type 2 diabetes (T2DM).
METHODS: Two questionnaires were designed for diabetes patients and their doctors. The questionnaires included the most important indicators for determining the patient's condition, the ability of TCM in treating T2DM, the length of time it takes forTCM to be effective, and when to adjust the prescription dosage. The frequency of answers was calculated, summarized, and analyzed after the survey.

Tuesday, 23 July 2013

Wogonoside induces cell cycle arrest and differentiation by affecting expression and subcellular localization of PLSCR1 in AML cells

Wogonoside induces cell cycle arrest and differentiation by affecting expression and subcellular localization of PLSCR1 in AML cells.
Blood. 2013 May 2;121(18):3682-91
Authors: Chen Y, Hui H, Yang H, Zhao K, Qin Y, Gu C, Wang X, Lu N, Guo Q
Abstract
Wogonoside is the main flavonoid component derived from the root of Scutellaria baicalensis Georgi. It is a popular Chinese herbal medicine with the potential to treat hematologic malignancies. In this study, we investigated the anticancer effects of wogonoside in acute myeloid leukemia (AML) cell lines and primary patient-derived AML cells.

Monday, 22 July 2013

Management of tremor in medieval Persia

Management of tremor in medieval Persia.
J Hist Neurosci. 2013;22(1):53-61
Authors: Zargaran A, Zarshenas MM, Mehdizadeh A, Mohagheghzadeh A
Abstract
Tremor has been described in traditional systems of medicine throughout history. Persian medicine was one of those systems in medieval times and in it neurology and neurosurgery were also widely practiced and accepted. Based on the main Persian medical manuscripts, the current study focuses on the medieval concept of tremor as an important neurological disorder in order to clarify the development of neurology. Accordingly, three main approaches to the control and treatment of tremor in traditional Persian medicine are considered. First is lifestyle modification. The administration of simple medicines is the second, and the last is the application of compound medicines. Our study shows how much was known about tremor in traditional Persian medicine.
PMID: 23323532 [PubMed - indexed for MEDLINE]

Sunday, 21 July 2013

The medical plant butterbur (Petasites): analytical and physiological (re)view

The medical plant butterbur (Petasites): analytical and physiological (re)view.
J Pharm Biomed Anal. 2013 Mar 5;75:220-9
Authors: Aydın AA, Zerbes V, Parlar H, Letzel T
Abstract








Butterbur










Butterbur (Petasites) is an ancient plant which has been used for medical and edible purposes with its spasmolytic agents. However, toxic alkaloid content of the plant limits its direct usage. The paper covers the pyrrolizidine alkaloids (PAs) and butterbur themes in detail in order to display the outline of alkaloid-free plant extract production for medical and edible purposes. The toxic PAs and medicinal constituents of the plant are described with emphasis on analytics, physiological effects and published patent data on alkaloid free extract production.

Saturday, 20 July 2013

Diabetic nephropathy: causes and managements

Diabetic nephropathy: causes and managements.
Recent Pat Endocr Metab Immune Drug Discov. 2013 Jan;7(1):57-64
Authors: Tripathi YB, Yadav D
Abstract
Uncontrolled diabetic patients often develop diabetic nephropathy (DN). Since, it involves different molecular pathways, such as polyol pathway, protein kinase-C (PKC) activation, inflammatory and oxidative stress etc., and so multi-targeted drugs would be required for its management. Although, combination therapies with various conventional medicines are already in use, but herbal preparations are being preferred as adjuvant therapy, because of their multi targeted drug action due to the presence of natural cocktail of various secondary metabolites. This review describes pathogenesis of DN, possible approaches for its management and information about recent patents and bio-products, which are in pipe line of drug development.
PMID: 23227864 [PubMed - indexed for MEDLINE]

Friday, 19 July 2013

Anti-allergic potential of herbs and herbal natural products - activities and patents

Anti-allergic potential of herbs and herbal natural products - activities and patents.
Recent Pat Endocr Metab Immune Drug Discov. 2013 Jan;7(1):26-56
Authors: Cota BB, Bertollo CM, de Oliveira DM
Abstract
The increase of allergic diseases has accompanied the global population growth and the major challenge is to reduce morbidity. The currently available treatments present limitations regarding efficacy and safety. Hence, patients with chronic allergic conditions seek alternatives to achieve better control of symptoms. Many natural products have been identified as potential anti-allergic agents. In addition, plant formulations have demonstrated, in general, to be safe in clinical trials and demonstrate additional effects along with Western medicines such as synergism and modulation of the immune system.

Thursday, 18 July 2013

The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India

BMC Genomics. 2013 Jul 4;14(1):445
Authors: Cook R, Hennell JR, Lee S, Khoo CS, Carles MC, Higgins VJ, Govindaraghavan S, Sucher NJ
Abstract
BACKGROUND: Pattern-oriented chemical profiling is increasingly being used to characterize the phytochemical composition of herbal medicines for quality control purposes. Ideally, a fingerprint of the biological effects should complement the chemical fingerprint. For ethical and practical reasons it is not possible to test each herbal extract in laboratory animals or humans. What is needed is a test system consisting of an organism with relevant biology and complexity that can serve as a surrogate in vitro system.

Wednesday, 17 July 2013

Application of herbal medicine alba in treatment of patients with the pathology of thyroid

Application of herbal medicine alba in treatment of patients with the pathology of thyroid].
Lik Sprava. 2012 Dec;(8):116-9
Authors: Kiselëva IA, Tëplaia EV, Kaminskiĭ AV
Abstract
High prevalence of hyperplastic and autoimmune diseases of thyroid in Ukrainian population is determined by endemic deficit of iodine and selenium. The aim of this research was to assess the place of biologically-active additions on the basis of herbal material containing an iodine and selenium in prophylaxis and treatment of thyroid pathology. During the six month period 55 patients received herbal preparation Alba twice a day. The levels of TSH, volume of thyroid, the sizes of nodular goiter (ultrasound investigation) were measured before and at the end of the investigation. The levels of thyroid stimulating antibodies to TSH receptor (AB-r TSH) were evaluated in patients with hyperthyroidism. The results of Alba application showed that in patients with thyroid pathology (diffuse nontoxic goiter, hyperthyroidism and chronic thyroiditis) it was possible to reduce the volume of thyroid, normalize its function, and decrease the level of AB-r TSH in diffuse toxic goiter. We also found approximately 20 % shortening of the time needed to get target level of TSH and finally the duration of treatment of thyrotoxicosis.
PMID: 23786024 [PubMed - indexed for MEDLINE]

Tuesday, 16 July 2013

Potential value of a rice protein extract, containing proteinaceous inhibitors against cysteine proteinases from Porphyromonas gingivalis, for managing periodontal diseases

Biosci Biotechnol Biochem. 2013;77(1):80-6
Authors: Taiyoji M, Yamanaka T, Tsuno T, Ohtsubo S
Abstract
Arg-specific gingipain (Rgp) is a major pathogenic determinant of Porphyromonas gingivalis which is a major pathogen in periodontal disease. We prepared protein extracts with Rgp-inhibitory activity from polished rice (Oryza sativa) and evaluated the effects of these extracts on the growth and pathogenicity of P. gingivalis.

Monday, 15 July 2013

Observation on clinical efficacy of activating renal blood circulation and ovarian stimulation formula in treating ovulation failure infertility

Observation on clinical efficacy of activating renal blood circulation and ovarian stimulation formula in treating ovulation failure infertility].
Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(1):119-22
Authors: Fan XD, Ma K, Shan J, Jin XT
Abstract
OBJECTIVE: To discuss the clinical efficacy of the activating renal blood circulation and ovarian stimulation formula in treating ovulation failure infertility.
METHOD: Eighty-six cases were randomly divided into two groups: the treatment group and the control group. The treatment group is administered with the activating renal blood circulation and ovarian stimulation formula (composed of 15 g Cuscutae Semen, 15 g Dipsaci Asperoidis Radix, 15 g Lycii Fructus, 15 g Spatholobi Caulis, 10 g Ligustri Lucidi Fructus, 15 g Lycopi Herba, 10 g Typhae Polleu, 10 g Angelicae Sinensis Radix, 15 g Cyathulae Radix etc.), whereas the control group was given clomiphene.
RESULT: The treatment group showed a pregnancy rate of 58.14%, with an ovulation rate of 68.6%. While the control group showed a pregnancy rate of 36.67%, with an ovulation rate of 70%.
CONCLUSION: The comparison between the two groups showed that the activating renal blood circulation and ovarian stimulation formula was significantly different from clomiphene in statistical analysis (P < 0.05), without notable difference in the ovulation rate. Before and after the treatment, there is no significant difference in diameter of dominant follicles between the two groups, with remarkable difference in endometrium (P < 0.05).
PMID: 23596887 [PubMed - indexed for MEDLINE

Sunday, 14 July 2013

Brewers' spent grain; bioactivity of phenolic component, its role in animal nutrition and potential for incorporation in functional foods: a review

Brewers' spent grain; bioactivity of phenolic component, its role in animal nutrition and potential for incorporation in functional foods: a review.
Proc Nutr Soc. 2013 Feb;72(1):117-25
Authors: McCarthy AL, O'Callaghan YC, Piggott CO, FitzGerald RJ, O'Brien NM
Abstract
Brewers' spent grain (BSG) is a low-value co-product of the brewing industry produced in large quantities annually. This paper reviews the existing evidence regarding the phenolic component of BSG, focusing on composition, extraction and biofunctions such as antioxidant, anti-atherogenic, anti-inflammatory and anti-carcinogenic activities.

Saturday, 13 July 2013

Synthetic cannabinoids and potential reproductive consequences

Synthetic cannabinoids and potential reproductive consequences.
Life Sci. 2013 Jul 1;
Authors: Sun X, Dey SK
Abstract
Increases in emergency room visits due to abuse of designer drugs, popularly known by the street names "K2" and "Spice," are a cause for social, judicial, and clinical concerns. The psychoactive components in these herbal drugs mainly consist of different synthetic cannabinoids, and users of these street drugs are primarily within the age group of 12 to 20 years old. The abusive use of synthetic cannabinoids results in anxiety, nausea, vomiting, tachycardia, elevated blood pressure, tremors, seizures, hallucinations, and paranoid behavior, but the effects of maternal use of synthetic cannabinoids during pregnancy are ambiguous due to limited studies in humans and a relative short history of the drugs.

Friday, 12 July 2013

Ginkgo biloba extract and long-term cognitive decline: a 20-year follow-up population-based study

Ginkgo biloba extract and long-term cognitive decline: a 20-year follow-up population-based study.
PLoS One. 2013;8(1):e52755
Authors: Amieva H, Meillon C, Helmer C, Barberger-Gateau P, Dartigues JF
Abstract
BACKGROUND: Numerous studies have looked at the potential benefits of various nootropic drugs such as Ginkgo biloba extract (EGb761®; Tanakan®) and piracetam (Nootropyl®) on age-related cognitive decline often leading to inconclusive results due to small sample sizes or insufficient follow-up duration. The present study assesses the association between intake of EGb761® and cognitive function of elderly adults over a 20-year period.

Thursday, 11 July 2013

Metabolomic study of biochemical changes in the plasma and urine of primary dysmenorrhea patients using UPLC-MS coupled with a pattern recognition approach

Metabolomic study of biochemical changes in the plasma and urine of primary dysmenorrhea patients using UPLC-MS coupled with a pattern recognition approach.
J Proteome Res. 2013 Feb 1;12(2):852-65
Authors: Su S, Duan J, Wang P, Liu P, Guo J, Shang E, Qian D, Tang Y, Tang Z
Abstract
Primary dysmenorrhea (PD) is characterized by painful menstrual cramps without any organic pathology and has a prevalence of up to 90% in adolescents. Recent advances in its etiology and pathogenesis are providing more speculative hypotheses focused on integral systems. Using a targeted tandem mass spectrometry (MS/MS)-based metabolomic platform, we explored the changes of metabolic profiling in plasma/urine simultaneously between PD patients and healthy controls before and after a 3-month herbal medicine (namely Shaofu Zhuyu formula concentrated-granule, SFZYFG) therapy.

Wednesday, 10 July 2013

Grape resveratrol increases serum adiponectin and downregulates inflammatory genes in peripheral blood mononuclear cells: a triple-blind, placebo-controlled, one-year clinical trial in patients with stable coronary artery disease

Grape resveratrol increases serum adiponectin and downregulates inflammatory genes in peripheral blood mononuclear cells: a triple-blind, placebo-controlled, one-year clinical trial in patients with stable coronary artery disease.
Cardiovasc Drugs Ther. 2013 Feb;27(1):37-48
Authors: Tomé-Carneiro J, Gonzálvez M, Larrosa M, Yáñez-Gascón MJ, García-Almagro FJ, Ruiz-Ros JA, Tomás-Barberán FA, García-Conesa MT, Espín JC
AbstractPURPOSE: The grape and wine polyphenol resveratrol exerts cardiovascular benefits but evidence from randomized human clinical trials is very limited. We investigated dose-depending effects of a resveratrol-containing grape supplement on stable patients with coronary artery disease (CAD) treated according to currently accepted guidelines for secondary prevention of cardiovascular disease.

Tuesday, 9 July 2013

Efficacy of porcine placental extract on climacteric symptoms in peri- and postmenopausal women

Efficacy of porcine placental extract on climacteric symptoms in peri- and postmenopausal women.
Climacteric. 2013 Feb;16(1):28-35
Authors: Koike K, Yamamoto Y, Suzuki N, Yamazaki R, Yoshikawa C, Takano F, Takuma K, Sugiura K, Inoue M
Abstract
OBJECTIVES: Injections of human placental extract have long been used to treat menopausal symptoms. Recently, porcine placental extract (PPE), an oral supplement, has been developed for this purpose. The aim of this study was to assess whether PPE has an impact on climacteric symptoms in perimenopausal and postmenopausal women.
METHODS: Seventy-six women with climacteric symptoms were enrolled into this open-label, randomized, controlled study.

Monday, 8 July 2013

Synergistic cytotoxicity of red beetroot (Beta vulgaris L.) extract with doxorubicin in human pancreatic, breast and prostate cancer cell lines.



Synergistic cytotoxicity of red beetroot (Beta vulgaris L.) extract with doxorubicin in human pancreatic, breast and prostate cancer cell lines.
J Complement Integr Med. 2013;10(1):1-10
Authors: Kapadia GJ, Rao GS, Ramachandran C, Iida A, Suzuki N, Tokuda H
Abstract
Abstract Although a wide variety of cytotoxic plant extracts and phytochemicals are known to act synergistically with anticancer drug doxorubicin (D), their clinical application is hindered by safety concerns of such combination therapy. Our earlier studies showed that red beetroot (Beta vulgaris L.) extract (B), approved by Food and Drug Administration and European Union as red food color E162, reduced multi-organ tumor formations in various animal models when administered in drinking water.

Sunday, 7 July 2013

Anticancer, antiobesity, and anti-inflammatory activity of Artemisia species in vitro

Anticancer, antiobesity, and anti-inflammatory activity of Artemisia species in vitro.
J Tradit Chin Med. 2013 Feb;33(1):92-7
Authors: Choi E, Park H, Lee J, Kim G
Abstract
OBJECTIVE: To investigate the anticancer, anti-inflammatory, and antiobesity activity of methanol extracts of eight distinct species: Artemisia Stolonifera (AST), Artemisia Selengensis (ASE), Artemisia Japonica, Artemisia Montana, Artemisia Capillaris (ACA), Artemisia Sylvatica (ASY), Artemisia Keiskeana (AKE), and Artemisia Scoparia (ASC) in vitro.
METHODS: Antiproliferative activity was investigated in human breast cancer estrogen receptor-a positive T47D and negative HS578T cell lines exposed to the extracts at various concentrations (5-200 mg/ mL) for 24, 48, and 72 h. For evaluating the anti-inflammatory activity of the extracts, inhibition of nitrite synthesis was investigated in lipopolysaccharide (LPS)-stimulated cultures of macrophages cells exposed to 10, 50, 100, and 200 mg/mL for 24 h.

Saturday, 6 July 2013

Terpenoids from Zingiber officinale (Ginger) induce apoptosis in endometrial cancer cells through the activation of p53.

Terpenoids from Zingiber officinale (Ginger) induce apoptosis in endometrial cancer cells through the activation of p53.

PLoS One. 2012;7(12):e53178
Authors: Liu Y, Whelan RJ, Pattnaik BR, Ludwig K, Subudhi E, Rowland H, Claussen N, Zucker N, Uppal S, Kushner DM, Felder M, Patankar MS, Kapur A
Abstract
Novel strategies are necessary to improve chemotherapy response in advanced and recurrent endometrial cancer. Here, we demonstrate that terpenoids present in the Steam Distilled Extract of Ginger (SDGE) are potent inhibitors of proliferation of endometrial cancer cells. SDGE, isolated from six different batches of ginger rhizomes, consistently inhibited proliferation of the endometrial cancer cell lines Ishikawa and ECC-1 at IC(50) of 1.25 µg/ml. SDGE also enhanced the anti-proliferative effect of radiation and cisplatin. Decreased proliferation of Ishikawa and ECC-1 cells was a direct result of SDGE-induced apoptosis as demonstrated by FITC-Annexin V staining and expression of cleaved caspase 3. GC/MS analysis identified a total of 22 different terpenoid compounds in SDGE, with the isomers neral and geranial constituting 30-40%. Citral, a mixture of neral and geranial inhibited the proliferation of Ishikawa and ECC-1 cells at an IC(50) 10 µM (2.3 µg/ml). Phenolic compounds such as gingerol and shogaol were not detected in SDGE and 6-gingerol was a weaker inhibitor of the proliferation of the endometrial cancer cells. SDGE was more effective in inducing cancer cell death than citral, suggesting that other terpenes present in SDGE were also contributing to endometrial cancer cell death. SDGE treatment resulted in a rapid and strong increase in intracellular calcium and a 20-40% decrease in the mitochondrial membrane potential. Ser-15 of p53 was phosphorylated after 15 min treatment of the cancer cells with SDGE. This increase in p53 was associated with 90% decrease in Bcl2 whereas no effect was observed on Bax. Inhibitor of p53, pifithrin-α, attenuated the anti-cancer effects of SDGE and apoptosis was also not observed in the p53(neg) SKOV-3 cells. Our studies demonstrate that terpenoids from SDGE mediate apoptosis by activating p53 and should be therefore be investigated as agents for the treatment of endometrial cancer.
PMID: 23300887 [PubMed - indexed for MEDLINE]
www.irishcollegeoftraditionalandintegrativemedicine.com

Friday, 5 July 2013

Evaluation of the effect of hydroxypropyl-β-cyclodextrin on topical administration of milk thistle extract

                                                                                                                                                         


Evaluation of the effect of hydroxypropyl-β-cyclodextrin on topical administration of milk thistle extract.
Carbohydr Polym. 2013 Jan 30;92(1):40-7
Authors: Spada G, Gavini E, Cossu M, Rassu G, Carta A, Giunchedi P
Abstract
Two water in oil emulsions composed by eudermic ingredients as glycerin, cocoa butter, almond oil and a variety of lipids, were enriched respectively with milk thistle dry extract (MT) or with a binary complex composed by MT and hydroxypropyl-β-cyclodextrin (HP) (1:4 w/w) correspondent to 1% (w/w) in sylimarine in order to obtain two different emulsions designed for the skin delivery and determine influence of hydroxypropyl-β-cyclodextrin on the extract delivery and permeation. Uv-vis spectrophotometric analyses demonstrated that phytocomplex formation influences the finding of MT after the complexation process and the in vitro antioxidant activity. Further in vitro and ex vivo experiments demonstrated that the penetration capability of MT from formulations is strictly influenced by the phytocomplex able to control MT permeation; moreover phytocomplex increases flavonoids stability during the in vitro tests. Additionally, in vivo studies showed that the penetration into the stratum corneum of the active ingredients is effectively achieved by the phytocomplex formation, in fact about 80% of MT is absorbed by the skin along 1h despite the 30% of MT not complexed absorbed during the same period.
PMID: 23218263 [PubMed - indexed for MEDLINE]

Thursday, 4 July 2013

Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo.
Mol Biol Rep. 2013 Jan;40(1):597-604
Authors: Tepe B, Tuncer E, Saraydın SU, Ozer H, Sen M, Karadayi K, Inan DS, Karadayi S, Polat Z, Akpulat A, Duman M, Koksal B, Turan M
Abstract
This work aims to investigate the antiproliferative properties of Allium sivasicum (AS) on breast cancer. AS extracts were studied for cytotoxicity against the breast cancer cell lines. In vitro apoptosis studies of breast cancer cells were performed by annexin V staining in flow cytometry analyses. AS showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in AS treated cell lines were higher than the untreated control cells. The expressions of caspase-7 protein and TUNEL positive cells were much higher for the rats treated by AS, compared with the untreated control group.

Wednesday, 3 July 2013

Traditionally used Veronica officinalis inhibits proinflammatory mediators via the NF-κB signalling pathway in a human lung cell line.
J Ethnopharmacol. 2013 Jan 9;145(1):118-26
Authors: Gründemann C, Garcia-Käufer M, Sauer B, Stangenberg E, Könczöl M, Merfort I, Zehl M, Huber R
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Extracts from Veronica officinalis L. are traditionally used for the treatment of lung diseases; however, the effective compounds and the mode of action are still unknown.
AIM OF THE STUDY: Here we analyzed the effects of a standardized Veronica extract on genes expression and signalling protein production associated with the development of inflammatory lung diseases.

Tuesday, 2 July 2013

Black tea and blood pressure



Black tea lowers the rate of blood pressure variation: a randomized controlled trial.
Am J Clin Nutr. 2013 May;97(5):943-50
Authors: Hodgson JM, Croft KD, Woodman RJ, Puddey IB, Fuchs D, Draijer R, Lukoshkova E, Head GA
Abstract
BACKGROUND: Measures of blood pressure variation have been associated with cardiovascular disease and related outcomes. The regular consumption of black tea can lower blood pressure, but its effects on blood pressure variation have yet to be investigated.
OBJECTIVE: We aimed to assess the effects of black tea consumption on the rate of ambulatory blood pressure variation.

Monday, 1 July 2013

Peppermint oil and IBS

Efficacy of Peppermint oil in diarrhea predominant IBS - a double blind randomized placebo - controlled study.
Mymensingh Med J. 2013 Jan;22(1):27-30
Authors: Alam MS, Roy PK, Miah AR, Mollick SH, Khan MR, Mahmud MC, Khatun S
www.irishcollegeoftraditionalandintegrativemedicine.com
http://www.ibs-symptoms.ie/?gclid=CLKJkqq6i7gCFY4q2wodElcA9w
Abstract
Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorder which is associated with considerable sufferings of patient and Peppermint oil is volatile oil, its active principle is menthol-contain a cyclic monoterpine which has anti-spasmotic properties due to its ability to block calcium channel of intestinal smooth muscles.